製品の概要

  • 製品名Anti-GAPDH antibody
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAPDH
  • 特異性We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Gerbil, Chinese Hamster
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 300 and the C-terminus (residue 335) of Mouse GAPDH. (NP_001001303.1).

  • ポジティブ・コントロール
    • Whole cell lysate from mouse NIH3T3 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • 配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • 翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Information by UniProt
    • 参照データベース
    • 別名
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      • Glyceraldehyde 3 phosphate dehydrogenase antibody
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      • HEL-S-162eP antibody
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    Anti-GAPDH antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse squamous cell carcinoma tissue labelling GAPDH with ab70700 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-GAPDH antibody (ab70700) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from mouse NIH3T3 cells. at 50 µg
      Lane 2 : Whole cell lysate from mouse NIH3T3 cells. at 15 µg
      Lane 3 : Whole cell lysate from mouse NIH3T3 cells. at 5 µg


      Predicted band size : 36 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    Anti-GAPDH antibody (ab70700) 使用論文

    ab70700 has not yet been referenced specifically in any publications.

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