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DAPMFVMGVNHFKYA, corresponding to amino acids 126-140 of Human GAPDH.
Our Abpromise guarantee covers the use of ab22555 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa.|
ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.