製品の概要

  • 製品名Anti-GAPDH antibody
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to GAPDH
  • 特異性Ab14247 recognises GAPDH.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Protein domain corresponding to amino acids 51-290 of GAPDH.

  • ポジティブ・コントロール
    • 293 cell lysate.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab14247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 参照データベース
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Anti-GAPDH antibody 画像



  • Predicted band size : 36 kDa


    293 cell lysate as test antigen. Ab14247 dilution: 1:2000. Colorimetric method for signal development.

Anti-GAPDH antibody (ab14247) 使用論文

This product has been referenced in:
  • Moeton M  et al. GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions. Cell Mol Life Sci 73:4101-20 (2016). ICC/IF ; Human . Read more (PubMed: 27141937) »
  • Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015). WB . Read more (PubMed: 26403541) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"