Anti-GAP43 抗体 - Neuronal Marker (ab12274)

製品の概要

  • 製品名Anti-GAP43 antibody - Neuronal Marker
    GAP43 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAP43 - Neuronal Marker
  • アプリケーション適用あり: IHC-P, WB, ICC, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cat, Human, Fish, Monkey
  • 免疫原

    GAP43 purified from cat brain.

  • ポジティブ・コントロール
    • ICC/IF: SKNSH cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Detects a band of approximately 43-57 kDa (predicted molecular weight: 24 kDa).
ICC 1/100 - 1/400.
IP Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/400.

ターゲット情報

  • 機能This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • 配列類似性Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • 翻訳後修飾Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • 細胞内局在Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

Anti-GAP43 antibody - Neuronal Marker 画像

  • ICC/IF image of ab12274 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12274, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM



  • Predicted band size : 24 kDa

    This image is courtesy of an anonymous Abreview

    Western blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control). Western blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control).

    See Abreview

  • Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-GAP43 antibody - Neuronal Marker (ab12274) 使用論文

This product has been referenced in:
  • Falk DJ  et al. Peripheral nerve and neuromuscular junction pathology in Pompe disease. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 25217571) »
  • Lu YY  et al. TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death. Nat Commun 4:2852 (2013). WB ; Mouse . Read more (PubMed: 24284943) »

See all 7 Publications for this product

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Wie versprochen, habe ich mir die Abbildung 1 angesehen und daraufhin unsere GAP43- Antikörper danach durchsucht, welche für Gefrierschnitte und Par...

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Application Immunohistochemistry (Frozen sections)
Sample Xenopus laevis Tissue sections (Spinal cord (on sections of whole tadpole))
Permeabilization Yes - 0.2% Triton-X/100
Specification Spinal cord (on sections of whole tadpole)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
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投稿 Jan 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
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投稿 Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
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投稿 Nov 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain)
Loading amount 10 µg
Specification brain
Gel Running Conditions Reduced Denaturing (12% SDS)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Nov 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain, Sciatic Nerve)
Loading amount 20 µg
Specification Brain, Sciatic Nerve
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
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投稿 Jul 04 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"