製品の概要

  • 製品名
    GANT 61
  • 製品の詳細
    Selective GLI1 and 2 inhibitor
  • 別名
    • NSC 136476
  • 生理活性の詳細
    Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
  • 精製度
    > 98%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
    The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Pan D  et al. Gli inhibitor GANT61 causes apoptosis in myeloid leukemia cells and acts in synergy with rapamycin. Leuk Res 36:742-8 (2012). Read more (PubMed: 22398221) »
  • Barsoum I & Yao HH Redundant and differential roles of transcription factors Gli1 and Gli2 in the development of mouse fetal Leydig cells. Biol Reprod 84:894-9 (2011). Read more (PubMed: 21209421) »
  • Lauth M  et al. Inhibition of GLI-mediated transcription and tumor cell growth by small-molecule antagonists. Proc Natl Acad Sci U S A 104:8455-60 (2007). Read more (PubMed: 17494766) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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