The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/100 - 1/400. (50% OD at 1/200, end point at 1/10000).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Gangliosides are neuraminic acid containing glycosphingolipids and represent characteristic constituents of the plasma membrane of eukaryotic cells. They are shed in the microenvironment and found as free components in plasma. In turn, free gangliosides are efficiently incorporated into the plasma cell membrane.