Anti-gamma Tubulin 抗体 [TU-30] (ab27074)
Key features and details
- Mouse monoclonal [TU-30] to gamma Tubulin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-gamma Tubulin antibody [TU-30]
gamma Tubulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TU-30] to gamma Tubulin -
由来種
Mouse -
特異性
This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig, Plants -
免疫原
Synthetic peptide:
EYHAATRPDYISWGTQ
conjugated to KLH, corresponding to amino acids 434-449 of gamma Tubulin. -
エピトープ
This antibody reacts with an epitope located in the C terminal region of gamma tubulin. -
ポジティブ・コントロール
- ICC/IF: P19C1 mouse embryonal carcinoma cells; Mouse fibroblasts; HEK293T cells; Rat prostate carcinoma cells. Flow Cyt (Intra): HeLa cells; MCF-7 cells. WB: Jurkat, HeLa, HEK293T, U87MG all with reducing conditions.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This antibody was purified from hybridoma culture supernatant by affinity chromatography. Purity is > 95% (by SDS-PAGE). -
ポリ/モノ
モノクローナル -
クローン名
TU-30 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27074の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
reducing conditions. |
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ICC/IF | (1) |
Use a concentration of 1 - 2 µg/ml.
Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells. |
特記事項 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa. reducing conditions. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
別名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
画像
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Separation of MCF-7 cells stained using ab27074 (concentration in sample 9 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
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Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Western blotting analysis of gamma-tubulin using ab27074 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of ab27074 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.
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Immunocytochemisrty analysis of murine fibroblasts labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Nuclei were stained with DAPI (blue).
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Immunocytochemisrty analysis of 3T3 mouse fibroblasts in metaphase (A), anaphase (B) and telophase (C), labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Cells were costained for alpha-tubulin (green). Nuclei were stained with DAPI (blue).
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ab27074 staining gamma tubulin in the Human HEK293T cells by ICC. Cells were fixed with paraformaldehyde, permeabilized with methanol (-20ºC 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.
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ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC. The cells were also transfected with beta-tubulin GFP which stained green.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab27074 は 57 報の論文で使用されています。
- Singh A et al. BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms. Oncogene 42:572-585 (2023). PubMed: 36550359
- Würtz M et al. Modular assembly of the principal microtubule nucleator γ-TuRC. Nat Commun 13:473 (2022). PubMed: 35078983
- Clemm von Hohenberg K et al. Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division. Nat Commun 13:668 (2022). PubMed: 35115540
- Singh CK et al. PLK4 is upregulated in prostate cancer and its inhibition reduces centrosome amplification and causes senescence. Prostate 82:957-969 (2022). PubMed: 35333404
- Dang H et al. Centrosome linker protein C-Nap1 maintains stem cells in mouse testes. EMBO Rep 23:e53805 (2022). PubMed: 35599622