製品の概要

  • 製品名
    Anti-gamma Tubulin antibody [TU-30]
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TU-30] to gamma Tubulin
  • 特異性
    This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain.
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Chicken, Human, Pig, Xenopus laevis, Chinese hamster
    交差が予測される動物種: Plants
  • 免疫原

    Synthetic peptide:

    EYHAATRPDYISWGTQ

    conjugated to KLH, corresponding to amino acids 434-449 of gamma Tubulin.

  • エピトープ
    This antibody reacts with an epitope located in the C terminal region of gamma tubulin.
  • ポジティブ・コントロール
    • P19 cell line, 3T3 cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 15mM Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    This antibody was purified from hybridoma culture supernatant by affinity chromatography. Purity is > 95% (by SDS-PAGE).
  • ポリ/モノ
    モノクローナル
  • クローン名
    TU-30
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 2 µg/ml.

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能
    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 配列類似性
    Belongs to the tubulin family.
  • 翻訳後修飾
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

画像

  • Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

  • ab27074 staining gamma tubulin in human colon and thyroid tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in EDTA pH 9.0 without undergoing blocking step. The primary antibody was diluted 1/1000 and incubated with sample for 1 hour at room temperature. A HRP-conjugated goat polyclonal to mouse antibody was used undiluted, as secondary.

    See Abreview

  • ab27074 staining gamma tubulin in the Human HEK293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol (-20ºC 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC/IF. The cells were also transfected with beta-tubulin GFP which stained green.

  • Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Denu RA  et al. Centrosome amplification induces high grade features and is prognostic of worse outcomes in breast cancer. BMC Cancer 16:47 (2016). IHC . Read more (PubMed: 26832928) »
  • Wike CL  et al. Excess free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans. Cell Cycle 15:2216-2225 (2016). Read more (PubMed: 27248858) »

See all 18 Publications for this product

レビューと Q&A

Thank you for contacting us. This product has been tested for Immunohistochemistry. The reference is as follows:

Nicholas AK et al. WDR62 is associated with the spindle pole and is mutated in human microcephaly. Nat Genet 42:1010-4 (2010). IHC...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T)
Specification
HEK 293T
Fixative
Paraformaldehyde
Permeabilization
Yes - Methanol -20ºC 20 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon and Thyroid)
Specification
Colon and Thyroid
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9.0 EDTA
Username

Mr. 孝一 鎌田

Verified customer

投稿 Dec 11 2008

Thank you for your enquiry. I can tell you that this antibody has been applied in the following plant publication; Binarova P et al. Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell 12:433-42 (2000). PubMed: 10715328 I have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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