製品の概要

  • 製品名
    Anti-gamma Tubulin antibody [GTU-88]
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GTU-88] to gamma Tubulin
  • アプリケーション
    適用あり: IHC-P, ICC, ELISA, WB, IHC-FrFl, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis, Indian muntjac
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 20890279
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18786414

ターゲット情報

Anti-gamma Tubulin antibody [GTU-88] 画像

  • All lanes : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/10000 dilution

    Lane 1 : HeLa cell line lysates
    Lane 2 : U87 cell line lysates
    Lane 3 : COS7 cell line lysates
    Lane 4 : P19 cell line lysates
    Lane 5 : RAT19 cell line lysates
    Lane 6 : CHO cell line lysates
    Lane 7 : MDBK cell line lysates
    Lane 8 : MDCK cell line lysates

    Secondary
    Goat Anti-Mouse IgG-Peroxidase

    Predicted band size : 48 kDa
  • ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

    This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

    See Abreview

  • Lane 1 : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/20000 dilution
    Lane 2 : Negative control (secondary antibody only)

    Lane 1 : Whole cell extract of chicken fibroblasts
    Lane 2 : Whole cell extract of chicken fibroblasts

    Secondary
    Alkaline Phosphatase conjugated goat anti-mouse IgG

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

Anti-gamma Tubulin antibody [GTU-88] (ab11316) 使用論文

This product has been referenced in:
  • Morris EJ  et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nat Commun 8:15289 (2017). WB, FM ; Apteronotus leptorhynchus . Read more (PubMed: 28474672) »
  • Kime C  et al. Autotaxin-mediated lipid signaling intersects with LIF and BMP signaling to promote the naive pluripotency transcription factor program. Proc Natl Acad Sci U S A 113:12478-12483 (2016). WB . Read more (PubMed: 27738243) »

See all 56 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Nov 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29)
Specification
HT29
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% TritonX100 in PBS
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
30 µg
Specification
HCT116
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Abcam user community

Verified customer

投稿 Jun 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
10 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 01 2012

DISCOUNT CODE: XXXXXX-XXXXX Expiration date: DD MM 20YY Thank you very much for your interest in ab11316. To our knowledge, ab11316 has not been tested in Zebrafish. I am very pleased to hear you would like to accept our off...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain, ependymal cells lining the ventricular wall)
Specification
Brain, ependymal cells lining the ventricular wall
Username

Mr. Karthikeyan Devaraju

Verified customer

投稿 Jun 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Liver cancer cell line)
Specification
Liver cancer cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
methanol/acetone
Permeabilization
Yes - air dry
Blocking step
FCS as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: RT°C
Username

Dr. Ioannis Gavvovidis

Verified customer

投稿 Dec 02 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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