製品の概要

  • 製品名Anti-gamma Tubulin antibody [GTU-88]
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GTU-88] to gamma Tubulin
  • アプリケーション適用あり: IHC-P, ICC, ELISA, WB, IHC-FrFl, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis, Indian Muntjac
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 20890279

Use at an assay dependent concentration.

ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Use at an assay dependent concentration.

WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-FrFl Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF Use at an assay dependent concentration.

Use at an assay dependent concentration.

IHC-FoFr Use at an assay dependent concentration. PubMed: 18786414

Use at an assay dependent concentration.

ターゲット情報

Anti-gamma Tubulin antibody [GTU-88] 画像

  • All lanes : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/10000 dilution

    Lane 1 : HeLa cell line lysates
    Lane 2 : U87 cell line lysates
    Lane 3 : COS7 cell line lysates
    Lane 4 : P19 cell line lysates
    Lane 5 : RAT19 cell line lysates
    Lane 6 : CHO cell line lysates
    Lane 7 : MDBK cell line lysates
    Lane 8 : MDCK cell line lysates

    Secondary
    Goat Anti-Mouse IgG-Peroxidase

    Predicted band size : 48 kDa
  • ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

    This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

    See Abreview

  • Lane 1 : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/20000 dilution
    Lane 2 : Negative control (secondary antibody only)

    Lane 1 : Whole cell extract of chicken fibroblasts
    Lane 2 : Whole cell extract of chicken fibroblasts

    Secondary
    Alkaline Phosphatase conjugated goat anti-mouse IgG

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

Anti-gamma Tubulin antibody [GTU-88] (ab11316) 使用論文

This product has been referenced in:
  • Da Costa R  et al. Localizing the RPGR protein along the cilium: a new method to determine efficacies to treat RPGR mutations. Gene Ther 22:413-20 (2015). Read more (PubMed: 25630948) »
  • Dumoux M  et al. A Chlamydia effector recruits CEP170 to reprogram host microtubule organization. J Cell Sci 128:3420-34 (2015). Human . Read more (PubMed: 26220855) »

See all 49 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment 100 ng/ml LPS
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Nov 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT29)
Specification HT29
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% TritonX100 in PBS
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 30 µg
Specification HCT116
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Abcam user community

Verified customer

投稿 Jun 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 10 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 01 2012

DISCOUNT CODE: XXXXXX-XXXXX Expiration date: DD MM 20YY Thank you very much for your interest in ab11316. To our knowledge, ab11316 has not been tested in Zebrafish. I am very pleased to hear you would like to accept our off...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (Brain, ependymal cells lining the ventricular wall)
Specification Brain, ependymal cells lining the ventricular wall
Username

Mr. Karthikeyan Devaraju

Verified customer

投稿 Jun 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Liver cancer cell line)
Specification Liver cancer cell line
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative methanol/acetone
Permeabilization Yes - air dry
Blocking step FCS as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: RT°C
Username

Dr. Ioannis Gavvovidis

Verified customer

投稿 Dec 02 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"