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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human gamma Tubulin.
(Peptide available as ab17097.)
ab84355 is raised using the same immunogen as ab16504. Batches sold under ab84355 have passed QC testing in Western Blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.
Our Abpromise guarantee covers the use of ab84355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||1/200. Please note that this antibody only works in ICC/IF if used in conjunction with methanol-fixed cells.|
gamma Tubulin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to gamma Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84355.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 48kDa; gamma Tubulin
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"