Anti-gamma Tubulin 抗体 - Centrosome Marker (ab84355)


  • 製品名Anti-gamma Tubulin antibody - Centrosome Marker
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Tubulin - Centrosome Marker
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human gamma Tubulin.

    (Peptide available as ab17097.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat, NIH 3T3
  • 特記事項

    ab84355 is raised using the same immunogen as ab16504. Batches sold under ab84355 have passed QC testing in Western Blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.



Our Abpromise guarantee covers the use of ab84355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use a concentration of 5 µg/ml.
ICC/IF 1/200. Please note that this antibody only works in ICC/IF if used in conjunction with methanol-fixed cells.


Anti-gamma Tubulin antibody - Centrosome Marker 画像

  • gamma Tubulin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to gamma Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84355.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 48kDa; gamma Tubulin

  • All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab84355) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab84355 (1/200) staining gamma tubulin in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.

    See Abreview

Anti-gamma Tubulin antibody - Centrosome Marker (ab84355) 使用論文

This product has been referenced in:
  • Zhu YT  et al. Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells. J Cell Biol 206:799-811 (2014). Human . Read more (PubMed: 25202030) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10%
Sample Dog Cell (MDCK)
Specification MDCK
Permeabilization No
Fixative Methanol

Abcam user community

Verified customer

投稿 Jun 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No

Dr. Kirk McManus

Verified customer

投稿 Oct 14 2010