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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human gamma Tubulin.
(Peptide available as ab17097.)
Our Abpromise guarantee covers the use of ab16504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Abcam recommends using milk as the blocking agent.
Lane 1 - 6 : gamma Tubulin antibody - Centrosome Marker (ab11317) at 1 ug/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 5 : HeLa Nuclear Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 6 : A431 Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lysates at 20 ug.
Blocking peptide at 1 ug/ml.
Performed under reducing conditions.
Observed band size : 48kD
ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.
Paraformaldehyde-fixed mouse embryo tissue stained for gamma Tubulin (red) using ab16504 in immunohistochemical analysis.
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.