Synthetic peptide from within residues: GSYLLERLND RYPKKLVQTY SVFPNQDEMS DVVVQPYNSL LTLKRLTQNA D, corresponding to amino acids 150-200 of Human gamma Tubulin using the numbering given in entry NP_001061.2.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Belongs to the tubulin family.
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
3µg ab93867 were used to immunoprecipitate gamma Tubulin from 3µg HeLa whole cell lysate. 20% IP was loaded on the gel and probed with a different anti-gamma Tubulin antibody. Detection: Chemiluminescence with an exposure time of 3 seconds.