製品の概要

  • 製品名Anti-gamma Tubulin antibody
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Tubulin
  • 特異性This sequence is highly specific for C. elegans gamma tubulin, and is not found in gamma tubulin sequences of other species or other members of the tubulin family such as alpha, beta, delta and epsilon tubulins.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Caenorhabditis elegans
  • 免疫原

    Synthetic peptide

    LDEYKAVVQKDYLTRGL

    with N-terminal added cysteine conjugated to KLH, corresponding to C terminal amino acids 428-444 of Caenorhabditis elegans gamma Tubulin

  • ポジティブ・コントロール
    • Whole extract of C. elegans

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50.
WB Use a concentration of 0.75 - 1.5 µg/ml. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 別名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody 画像

  • All lanes : Anti-gamma Tubulin antibody (ab50721) at 1/2000 dilution

    Lane 1 : C. elegans whole extract
    Lane 2 : C. elegans whole extract with gamma Tubulin immunizing peptide at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

Anti-gamma Tubulin antibody (ab50721) 使用論文

This product has been referenced in:
  • Wiese M  et al. Regulation of neuronal APL-1 expression by cholesterol starvation. PLoS One 7:e32038 (2012). WB ; Caenorhabditis elegans . Read more (PubMed: 22363792) »
  • Wiese M  et al. Intracellular trafficking and synaptic function of APL-1 in Caenorhabditis elegans. PLoS One 5: (2010). WB ; Caenorhabditis elegans . Read more (PubMed: 20862215) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (embryo)
Specification embryo
Fixative Paraformaldehyde
Permeabilization Yes - freeze-cracking and methanol (5 min at -20C)
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投稿 Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"