製品の概要

  • 製品名Anti-gamma Tubulin antibody
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Tubulin
  • 特異性Does not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Chicken, Hamster, Human, African Green Monkey, Tribolium castaneum
    交差が予測される動物種: Drosophila melanogaster, Schizosaccharomyces pombe, Sea Urchin
  • 免疫原

    Synthetic peptide:

    EEFATEGTDRKDVFFY-C

    conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin

  • ポジティブ・コントロール
    • A431 cultured cells extract; cultured chicken fibroblasts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/5000.

It is recommended to fix cells with methanol/acetone.

WB 1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Anti-gamma Tubulin antibody 画像

  • Ab11317 staining cultured hamster cells (CHO cells) by ICC. The cells were fixed with paraformaldehyde and incubated with ab11317 at a dilution of 1/5000 for 12 hours. A polyclonal goat anti-rabbit antibody conjugated to Alexa Fluor ® 488 was used as the seconday antibody.

    This image is courtesy of an Abreview submitted by Dr Jan Postberg on 7 December 2005.

    See Abreview

  • ab11317 at a 1/1000 dilution detecting gamma tubulin from human HeLa cells by ICC. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-rabbit Alexa-Fluor ® 488.

    This image is courtesy of an anonymous Abreview submitted on 7 December 2005.

    See Abreview

Anti-gamma Tubulin antibody (ab11317) 使用論文

This product has been referenced in:
  • Van Itallie CM  et al. Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell-cell and cell-substrate adhesion. J Cell Sci 127:885-95 (2014). Read more (PubMed: 24338363) »
  • Wichmann C  et al. Activating c-KIT mutations confer oncogenic cooperativity and rescue RUNX1/ETO-induced DNA damage and apoptosis in human primary CD34+ hematopoietic progenitors. Leukemia N/A:N/A (2014). Read more (PubMed: 24897507) »

See all 19 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibroblast)
Permeabilization No
Specification Fibroblast
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1%
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Jun 22 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample African Green Monkey Cell lysate - whole cell (cos7)
Specification cos7
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 24 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Oct 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (Embryonic fibroblasts)
Specification Embryonic fibroblasts
Permeabilization No
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Jun 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Sample Mouse Tissue sections (Embryonic brain)
Specification Embryonic brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MIN6)
Loading amount 30 µg
Specification MIN6
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Specification fibroblast
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (IMCD3)
Specification IMCD3
Fixative Methanol
Permeabilization No
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 60 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS-7)
Loading amount 40 µg
Specification COS-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 29 2008

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"