製品の概要

  • 製品名
    Anti-gamma Tubulin antibody
    gamma Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Tubulin
  • 特異性
    Does not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2.
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Chicken, Hamster, Human, African green monkey, Tribolium castaneum
    交差が予測される動物種: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
  • 免疫原

    Synthetic peptide:

    EEFATEGTDRKDVFFY-C

    conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin

  • ポジティブ・コントロール
    • WB: A431, HeLa, Jurkat, C2C12, Rat2, U87, NIH3T3 and CHO cell lysates. ICC/IF: NIH3T3, HeLa and Rat2 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/5000.

It is recommended to fix cells with methanol/acetone.

WB 1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • 配列類似性
    Belongs to the tubulin family.
  • 翻訳後修飾
    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

画像

  • Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : Rat2 cell lysate
    Lane 6 : U87 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : CHO cell lysate

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

  • ab11317 staining gamma Tubulin in mouse embryonic brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in 1mM EDTA. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 20°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

参考文献

This product has been referenced in:
  • Li X  et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB . Read more (PubMed: 28361952) »
  • Noble JW  et al. Loukoumasomes Are Distinct Subcellular Structures from Rods and Rings and Are Structurally Associated with MAP2 and the Nuclear Envelope in Retinal Cells. PLoS One 11:e0165162 (2016). IHC-Fr ; Rat . Read more (PubMed: 27798680) »

See all 35 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Permeabilization
No
Specification
Fibroblast
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 1%
Fixative
Methanol
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投稿 Jun 22 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
African Green Monkey Cell lysate - whole cell (cos7)
Specification
cos7
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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投稿 Sep 24 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Oct 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Mouse Cell (Embryonic fibroblasts)
Specification
Embryonic fibroblasts
Permeabilization
No
Fixative
Methanol
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投稿 Jun 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Sample
Mouse Tissue sections (Embryonic brain)
Specification
Embryonic brain
Permeabilization
No
Fixative
Paraformaldehyde
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投稿 Jun 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MIN6)
Loading amount
30 µg
Specification
MIN6
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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投稿 Nov 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Specification
fibroblast
Fixative
Methanol
Permeabilization
No
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (IMCD3)
Specification
IMCD3
Fixative
Methanol
Permeabilization
No
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
60 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS-7)
Loading amount
40 µg
Specification
COS-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 29 2008

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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