製品の概要

  • 製品名
    Anti-gamma Synuclein antibody
    gamma Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Synuclein
  • 特異性
    ab55424 detects endogenous levels of total gamma Synuclein protein.
  • アプリケーション
    適用あり: IHC-Fr, WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human gamma Synuclein.

  • ポジティブ・コントロール
    • human lung carcinoma tissue and HT29 cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • 組織特異性
    Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • 配列類似性
    Belongs to the synuclein family.
  • 翻訳後修飾
    Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • 細胞内局在
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCSG1 antibody
    • Breast cancer specific gene 1 protein antibody
    • Breast cancer specific protein 1 antibody
    • Breast cancer-specific gene 1 protein antibody
    • Gamma synuclein antibody
    • Gamma-synuclein antibody
    • Persyn antibody
    • PRSN antibody
    • SNCG antibody
    • SR antibody
    • Synoretin antibody
    • Synuclein gamma antibody
    • synuclein, gamma (breast cancer-specific protein 1) antibody
    • SYUG_HUMAN antibody
    see all

画像

  • ab55424 staining gamma Synclein in Rat retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized by freezing and blocked with 2% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in 2% Triton + 2% Serum in PBS) for 8 hours at 4°C. An Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab55424 (1/50 dilution), in the presence or absence of the immunizing peptide.
  • All lanes : Anti-gamma Synuclein antibody (ab55424) at 1/500 dilution

    Lane 1 : HT29 cell extract, without immunizing peptide
    Lane 2 : HT29 cell extract, with immunizing peptide


    Predicted band size : 13 kDa
    Observed band size : 13 kDa
  • ab55424 staining gamma synuclein in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in P) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab55424 staining gamma Synuclein in murine retinal tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • ab55424 staining gamma Synuclein in rat retinal tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab55424 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
  • Wang L  et al. Downregulation of microRNA-497 is associated with upregulation of synuclein ? in patients with osteosarcoma. Exp Ther Med 12:3761-3766 (2016). WB ; Human . Read more (PubMed: 28105108) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Dec 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Sample
Rat Cell (retina)
Specification
retina
Permeabilization
Yes - Freezing
Fixative
Formaldehyde
Username

Dra. Marta Agudo-Barriuso

Verified customer

投稿 Oct 10 2013

Thank you for your inquiry.

I heard back from the lab that the synthetic peptide used for production of this antibody is conjugated with KLH.



I hope this information helps. Please contact us with any other questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Retina)
Specification
Retina
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Retina)
Specification
Retina
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Aug 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Specification
Retina
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Aug 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Adipocyte)
Loading amount
5 µg
Specification
Adipocyte
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina samples)
Specification
mouse retina samples
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Kevin Jin

Verified customer

投稿 Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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