製品の概要

  • 製品名Anti-gamma Synuclein antibody [1H10D2]
    gamma Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H10D2] to gamma Synuclein
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Ni-NTA purified truncated recombinant human SNCG (a.a. 2-127) expressed in E. Coli strain BL21 (DE3)

  • ポジティブ・コントロール
    • Human ovarian carcinoma tissue, human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/2000.
ELISA 1/10000.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • 組織特異性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • 配列類似性Belongs to the synuclein family.
  • 翻訳後修飾Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • 細胞内局在Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCSG1 antibody
    • Breast cancer specific gene 1 protein antibody
    • Breast cancer specific protein 1 antibody
    • Breast cancer-specific gene 1 protein antibody
    • Gamma synuclein antibody
    • Gamma-synuclein antibody
    • Persyn antibody
    • PRSN antibody
    • SNCG antibody
    • SR antibody
    • Synoretin antibody
    • Synuclein gamma antibody
    • synuclein, gamma (breast cancer-specific protein 1) antibody
    • SYUG_HUMAN antibody
    see all

Anti-gamma Synuclein antibody [1H10D2] 画像

  • Anti-gamma Synuclein antibody [1H10D2] (ab47966) at 1/500 dilution + Truncated gamma Synuclein recombinant protein

    Predicted band size : 13 kDa
    Observed band size : 13 kDa
  • This image shows cytoplasmic staining of Human ovarian carcinoma tissue treated with ab47966 at 1/500 dilution.
  • This image shows cytoplasmic and nuclear staining of Human breast carcinoma tissue treated with ab47966 at 1/500 dilution.
  • Overlay histogram showing SH-SY5Y cells stained with ab47966 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47966, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-gamma Synuclein antibody [1H10D2] (ab47966) 使用論文

This product has been referenced in:
  • Boyer F & Dreyer JL The role of gamma-synuclein in cocaine-induced behaviour in rats. Eur J Neurosci 27:2938-51 (2008). WB, IHC-Fr ; Rat . Read more (PubMed: 18588534) »

See 1 Publication for this product

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