製品の概要

  • 製品名Anti-gamma Catenin antibody
    gamma Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gamma Catenin
  • 特異性Ab 15153 stains gamma catenin on the cell membrane and in the cytoplasm of breast carcinoma.
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) (C terminal).

  • ポジティブ・コントロール
    • Breast carcinoma

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 関連疾患Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
    Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • 配列類似性Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • 細胞内局在Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARVD 12 antibody
    • ARVD12 antibody
    • Catenin (cadherin associated protein), gamma 80kDa antibody
    • catenin (cadherin-associated protein) gamma (80kD) antibody
    • Catenin gamma antibody
    • CTNNG antibody
    • Desmoplakin 3 antibody
    • Desmoplakin III antibody
    • Desmoplakin-3 antibody
    • Desmoplakin3 antibody
    • DesmoplakinIII antibody
    • DP 3 antibody
    • DP III antibody
    • DP3 antibody
    • DPIII antibody
    • gamma catenin antibody
    • Junction plakoglobin antibody
    • JUP antibody
    • OTTHUMP00000164732 antibody
    • OTTHUMP00000164735 antibody
    • OTTHUMP00000164738 antibody
    • PDGB antibody
    • PKGB antibody
    • PLAK_HUMAN antibody
    • PLAKOGLOBIN antibody
    see all

Anti-gamma Catenin antibody 画像

  • Human breast carcinoma stained with anti gamma catenin antibody ab15153 (1/100 for 10 min at RT). Gamma catenin staining is seen on the cell membrane and in the cytoplasm.
  • ab15153 staining gamma Catenin in normal human skin tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% serum for 1 hour at 2°C; antigen retrival was by heat mediation in EDTA (pH9). The sample was incubated with primary antibody (1/100 in TBS + 1% BSA) at 21°C for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

  • ICC/IF image of ab15153 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-gamma Catenin antibody (ab15153) 使用論文

This product has been referenced in:
  • Guo Z  et al. E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci Signal 7:rs7 (2014). Read more (PubMed: 25468996) »
  • Rakha EA  et al. Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast. Am J Surg Pathol 34:1472-9 (2010). Read more (PubMed: 20871222) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Skin)
Specification Normal Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH 9
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 2°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2010

I can confirm that this antibody recognizes plakoglobin. Please let me know if you need further information,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"