製品の概要

  • 製品名
    Anti-gamma Actin antibody [2A3]
    gamma Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A3] to gamma Actin
  • アプリケーション
    適用あり: IHC-P, WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Synthetic peptide (Ac-

    EEEIAALVI

    -COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin

  • ポジティブ・コントロール
    • Human dermal fibroblast cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    2A3
  • ミエローマ
    NS0
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

ターゲット情報

  • 機能
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患
    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性
    Belongs to the actin family.
  • 翻訳後修飾
    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

画像

  • Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml

  • ab123034 at 1/500 dilution staining gamma Actin in methanol-fixed Human dermal fibroblasts by Immunofluorescence.

参考文献

This product has been referenced in:
  • Zou Y  et al. Characteristic expression and significance of CCL19 in different tissue types in chronic rhinosinusitis. Exp Ther Med 11:140-146 (2016). WB . Read more (PubMed: 26889230) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 27 2017

Yes , this antibody does not react with other isoforms and it binds to actin gamma 2 only.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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