Anti-gamma Actin 抗体 [2A3] (ab123034)
Key features and details
- Mouse monoclonal [2A3] to gamma Actin
- Suitable for: ELISA, IHC-Fr, WB, Flow Cyt, IHC-P, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
- Isotype: IgG2b
製品の概要
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製品名
Anti-gamma Actin antibody [2A3]
gamma Actin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A3] to gamma Actin -
由来種
Mouse -
特異性
This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.
This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
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アプリケーション
適用あり: ELISA, IHC-Fr, WB, Flow Cyt, IHC-P, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
交差が予測される動物種: a wide range of other species -
免疫原
Synthetic peptide (Ac-
EEEIAALVI
-COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin -
ポジティブ・コントロール
- ICC/IF: Human dermal fibroblast cells. IHC-P: Human small intestine and heart tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
2A3 -
ミエローマ
NS0 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
|
Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (2) |
1/100 - 1/500.
|
IHC-P |
Use at an assay dependent concentration.
|
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ICC/IF |
1/500.
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/500. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/500. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 415296 Chicken
- Entrez Gene: 71 Human
- Entrez Gene: 11465 Mouse
- Entrez Gene: 100361457 Rat
- Entrez Gene: 287876 Rat
- Entrez Gene: 57935 Zebrafish
- Omim: 102560 Human
- SwissProt: Q5ZMQ2 Chicken
see all -
別名
- ACT antibody
- ACTB antibody
- ACTG antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded human heart tissue immunostained for gamma cytoplasmic actin using ab123034 at 1/100 dilution.
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Human dermal fibroblasts stained for gamma Actin (green) using ab123034 at 1/500 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml
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ab123034 (1:1000 dilution) stainign gamma Actin in the epithelial villi and smooth muscle cells in swine colon.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab123034 は 16 報の論文で使用されています。
- Tang Y et al. Actin gamma 1 is a critical regulator of pancreatic ductal adenocarcinoma. Saudi J Gastroenterol 28:239-246 (2022). PubMed: 34856725
- Zhang L et al. Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1. Front Cell Dev Biol 9:644716 (2021). PubMed: 33791303
- Lee LL et al. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease. FEBS Open Bio 11:207-225 (2021). PubMed: 33135334
- Cui S et al. Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model. JOR Spine 4:e1146 (2021). PubMed: 34611583
- Yin C et al. Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections. J Anim Sci Biotechnol 11:71 (2020). PubMed: 32647569