Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T)
Specification
HEK 293T
Fixative
Paraformaldehyde
Permeabilization
Yes - Methanol -20ºC 20 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
45 minute(s) · Diluent: blocking solution 1:3 in PBSx1
Secondary antibody
Name
Non-Abcam antibody was used: Jackson anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Dilution
1/500
Additional data
Additional Notes
Excellent antibody! althought a slight nonspecific cytoplasmatic fluorescence is seen
Abcam response
Thank you very much for your feedback.
In order to reduce the cytoplasmtic background, we would like to suggest fixing the cells only in methanol for 10 min at 20°C to avoid overfixation.
In order to reduce the cytoplasmtic background, we would like to suggest fixing the cells only in methanol for 10 min at 20°C to avoid overfixation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 23 2010