Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
30 µg
Specification
U2OS cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Concentration
1 µg/ml
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 10 2007