Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Other product details
Concentration
5 µg/ml
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 chicken anti-rabbit
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
This antibody works fine on tissue sections, you can see very bright staining of mussles and some soft tissue (which is not what I wanted, but inevitable), but you can also see bright centrosome staining. So, it was Ok.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 30 2007