Application
Western blot
Sample
Mouse Cell lysate - whole cell (Adipocyte)
Loading amount
5 µg
Specification
Adipocyte
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking solution
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 13 kDa Non-specific: 100, 50, 30 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 15 2010