Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Trypsin
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
15 hour(s) and 30 minute(s) · Temperature: 23°C · Diluent: PBST
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotin SP-conj AffiniPure Donkey Anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin-maleimide
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin-maleimide
Additional data
Additional Notes
Tried multiple concentrations, 1:500, 1:750, 1:1000, 1:5000, but I was not able to see discrete puncti. Appeared to be staining the whole nucleus in multiple different tissue types (both irradiated and non-irradiated) in equal amounts.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 26 2016