Application
Western blot
Sample
Human Cell lysate - whole cell (Esophageal Tumor Cells)
Gel Running Conditions
Non-reduced Denaturing (12% SDS PAGE)
Loading amount
80 µg
Specification
Esophageal Tumor Cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Fluorescence Conjugated Secondary Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Detection
Detection method
Odyssey
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Additional data
Additional Notes
The gamma H2A.X in our study is used as a DNA damage marker. The image shows the gamma H2A.X in EC109 cells transfected with siRNAs. Although the antibody is a phosphorylated antibody, we blocked the antibody with 5% milk at 37C for 30 min - 1hour.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 31 2015