製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to GALR3
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    C-QGPEPREGPVHGGE

    , corresponding to C terminal amino acids 349-362 of Human GALR3.

  • ポジティブ・コントロール
    • Human skin and testes lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).

ターゲット情報

  • 機能
    Receptor for the hormone galanin.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GAL 3R antibody
    • GAL R3 antibody
    • GAL3 R antibody
    • GAL3-R antibody
    • GAL3R antibody
    • Galanin receptor 3 antibody
    • Galanin receptor family member 3 antibody
    • Galanin receptor type 3 antibody
    • GALN R3 antibody
    • GALNR 3 antibody
    • GALNR3 antibody
    • GALR 3 antibody
    • GALR-3 antibody
    • Galr3 antibody
    • GALR3_HUMAN antibody
    • OTTHUMP00000028820 antibody
    see all

画像

  • Anti-GALR3 antibody (ab48008) at 2 µg/ml + Human skin lysate in RIPA buffer at 35 µg/ml
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ab48008 (3.8µg/ml) staining of paraffin embedded Human Testis shows cellular membrane staining of spermatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

参考文献

ab48008 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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