製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to GALR1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    C-HLSDTKENKSRID

    by a Cysteine residue linker, corresponding to C terminal amino acids 327-339 of Human GALR1

  • ポジティブ・コントロール
    • Human Heart lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      The activity of this receptor for the hormone galanin is mediated by G proteins of the Gi/Go family that inhibit adenylate cyclase activity. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the colon, small intestine and heart. ESTs have been isolated from nerve and fetal lung/testis/B cell libraries.
    • 細胞内局在
      Cell membrane; Multi-pass membrane protein.
    • 参照データベース
    • 別名
      • gal receptor 1 antibody
      • gal-1 receptor antibody
      • GAL1R antibody
      • galanin receptor 1 antibody
      • galanin receptor 1, brain antibody
      • Galanin receptor antibody
      • Galanin receptor type 1 antibody
      • galanin-1 receptor antibody
      • GALNR antibody
      • GALNR1 antibody
      • GALR1 antibody
      • hugal 1-r antibody
      see all

    画像

    • Anti-GALR1 antibody (ab59025) at 0.1 µg/ml + Human Heart lysate (35µg protein in RIPA buffer)

      Predicted band size : 39 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
    • ab59025 staining GALR1 in Human small intestine tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with 0.015% for 30 mins at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-Goat polyclonal was used as the secondary antibody, diluted 1/200.

      See Abreview

    プロトコール

    参考文献

    ab59025 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Small intestine)
    Specification
    Small intestine
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.015% · Temperature: 25°C
    Username

    Dr. Karl Quint

    Verified customer

    投稿 Mar 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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