製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GALNT4
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cat, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 468-517 (TGANLSLFGC HGQGGNQFFE YTSNKEIRFN SVTELCAEVP EQKNYVGMQN) of human GALNT4 (NP_003765)

  • ポジティブ・コントロール
    • Fetal brain lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.
  • 組織特異性
    Ubiquitous. Highly expressed in mucous cells.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • ドメイン
    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.4.1.41 antibody
    • GALNAC T4 antibody
    • GalNAc transferase 4 antibody
    • GalNAc-T4 antibody
    • GalNAcT4 antibody
    • GALNT4 antibody
    • GALT4_HUMAN antibody
    • N-acetylgalactosaminyltransferase 4 (GalNAc-T4) antibody
    • Polypeptide GalNAc transferase 4 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • pp GaNTase 4 antibody
    • pp-GaNTase 4 antibody
    • Protein UDP acetylgalactosaminyltransferase 4 antibody
    • Protein-UDP acetylgalactosaminyltransferase 4 antibody
    • UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 antibody
    • UDP-N-acetyl-alpha-D-galactosamine:polypeptide antibody
    see all

画像

  • Anti-GALNT4 antibody (ab80676) at 1 µg/ml + fetal brain lysate at 10 µg

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : ~40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab80676 has not yet been referenced specifically in any publications.

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