製品の概要

  • 製品名Anti-GALNT2 antibody
    GALNT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GALNT2
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 32-62 (

    AGGAGGGAGRKEDWNEIDPIKKKDLHHSNGE

    ) of Human GALNT2

  • ポジティブ・コントロール
    • HeLa cell lysate. Human brain tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody is purified through a protein A column, followed by peptide affinity purification
  • ポリ/モノポリクローナル
  • アイソタイプIgG

アプリケーション

Our Abpromise guarantee covers the use of ab102650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50.

ターゲット情報

  • 機能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region.
  • 組織特異性Widely expressed.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • ドメインThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • 細胞内局在Golgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GalNAc T2 antibody
    • GalNAc-T2 antibody
    • Galnt2 antibody
    • GALT2_HUMAN antibody
    • GaNTase 2 antibody
    • Polypeptide GalNAc transferase 2 antibody
    • Polypeptide N acetylgalactosaminyltransferase 2 antibody
    • Polypeptide N acetylgalactosaminyltransferase 2 soluble form antibody
    • Polypeptide N-acetylgalactosaminyltransferase 2 soluble form antibody
    • pp GaNTase 2 antibody
    • pp-GaNTase 2 antibody
    • Protein UDP acetylgalactosaminyltransferase 2 antibody
    • Protein-UDP acetylgalactosaminyltransferase 2 antibody
    • UDP GalNAc transferase 2 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 2 (GalNAc T2) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 2 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 antibody
    see all

Anti-GALNT2 antibody 画像

  • ab102650 staining GALNT2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. A TRITC-conjugated goat anti-rabbit IgG monoclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab102650 staining GALNT2 in formalin fixed Human brain tissue at 1/50 dilution.
  • Anti-GALNT2 antibody (ab102650) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 65 kDa

Anti-GALNT2 antibody (ab102650) 使用論文

ab102650 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (HeLa cell)
Specification HeLa cell
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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投稿 Sep 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"