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Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 32-62 (
AGGAGGGAGRKEDWNEIDPIKKKDLHHSNGE) of Human GALNT2
Our Abpromise guarantee covers the use of ab102650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 65 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab102650 staining GALNT2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. A TRITC-conjugated goat anti-rabbit IgG monoclonal (1/250) was used as the secondary antibody.
ab102650 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"