製品の概要
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製品名Anti-GALNT12 antibody
GALNT12 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to GALNT12
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アプリケーション適用あり: WBmore details
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種交差性交差種: Mouse
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免疫原
Synthetic peptide corresponding to Mouse GALNT12 aa 550 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q8BGT9 -
ポジティブ･コントロール
- This antibody gave a positive signal in Mouse Pancreas tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab109917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
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機能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
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組織特異性Widely expressed at different levels of expression. Highly expressed in digestive organs such as small intestine, stomach, pancreas and colon. Expressed at intermediate level in testis, thyroid gland and spleen. Weakly expressed in whole brain, cerebral cortex, cerebellum, fetal brain, bone marrow, thymus, leukocytes, heart, skeletal muscle, liver, lung, esophagus, kidney, adrenal gland, mammary gland, uterus, placenta, ovary and prostate.
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パスウェイProtein modification; protein glycosylation.
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関連疾患Defects in GALNT12 are a cause of susceptibility to colorectal cancer type 1 (CRCS1) [MIM:608812]. Colorectal cancer is a malignancy originating either in the colon or rectum or both.
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配列類似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
ドメインThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
細胞内局在Golgi apparatus membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 230145 Mouse
- SwissProt: Q8BGT9 Mouse
- Unigene: 132246 Mouse
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別名
- Colorectal cancer susceptibility to 1 antibody
- CRCS1 antibody
- FLJ21212 antibody
see all
Anti-GALNT12 antibody 画像
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Western blot - Anti-GALNT12 antibody (ab109917)Anti-GALNT12 antibody (ab109917) at 1 µg/ml + Pancreas (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109917 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
プロトコール
Anti-GALNT12 antibody (ab109917) 使用論文
ab109917 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"