製品の概要

  • 製品名Anti-GALNT1 antibody
    GALNT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GALNT1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human GALNT1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa).

ターゲット情報

  • 機能Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
  • 組織特異性Widely expressed. Expressed in all tissues tested.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • ドメインThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
  • 細胞内局在Secreted and Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GALNAC T1 antibody
    • GalNAc transferase 1 antibody
    • GalNAc-T1 antibody
    • GALNT 1 antibody
    • GALNT1 antibody
    • GALT1_HUMAN antibody
    • Polypeptide GalNAc transferase 1 antibody
    • Polypeptide N acetylgalactosaminyltransferase 1 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
    • pp GaNTase 1 antibody
    • pp-GaNTase 1 antibody
    • Protein UDP acetylgalactosaminyltransferase 1 antibody
    • Protein-UDP acetylgalactosaminyltransferase 1 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
    see all

Anti-GALNT1 antibody 画像

  • All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa


    Exposure time : 20 minutes

Anti-GALNT1 antibody (ab109918) 使用論文

ab109918 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Loading amount 30 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Mar 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"