製品の概要

  • 製品名Anti-GALK1 antibody
    GALK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GALK1
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) from the N terminus of GALK1.

  • ポジティブ・コントロール
    • extracts from HUVEC cells and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Major enzyme for galactose metabolism.
  • パスウェイCarbohydrate metabolism; galactose metabolism.
  • 関連疾患Defects in GALK1 are the cause of galactosemia II (GALCT2) [MIM:230200]. Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.
  • 配列類似性Belongs to the GHMP kinase family. GalK subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.1.6 antibody
    • epididymis secretory protein Li 19 antibody
    • Galactokinase 1 antibody
    • Galactokinase antibody
    • Galactose kinase antibody
    • GALK 1 antibody
    • GALK antibody
    • GALK1 antibody
    • GALK1_HUMAN antibody
    • GK1 antibody
    • GLK antibody
    • GLK1 antibody
    • HEL-S-19 antibody
    see all

Anti-GALK1 antibody 画像

  • All lanes : Anti-GALK1 antibody (ab65123) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HeLa cells
    Lane 3 : extracts from HeLa cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GALK1 antibody (ab65123) 使用論文

ab65123 has not yet been referenced specifically in any publications.

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