製品の概要

  • 製品名Anti-galectin 9 antibody
    galectin 9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to galectin 9
  • 特異性This antibody detects endogenous levels of total Galectin-9 protein.
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human galectin 9 aa 51-100 (internal sequence).
    Database link: O00182

  • ポジティブ・コントロール
    • NIH/3T3 cells. Extracts from HepG2 cells, human lymphoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • 組織特異性Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • 配列類似性Contains 2 galectin domains.
  • ドメインContains two homologous but distinct carbohydrate-binding domains.
  • 細胞内局在Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 36 kDa beta-galactoside-binding lectin antibody
    • Ecalectin antibody
    • Gal-9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • UAT antibody
    • Urate transporter/channel antibody
    • Urate transporter/channel protein antibody
    see all

Anti-galectin 9 antibody 画像

  • All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa


    Exposure time : 30 seconds
    Ab69630 recognizes the partial tagged recombinant Human galectin 9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
  • ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.

Anti-galectin 9 antibody (ab69630) 使用論文

This product has been referenced in:
  • Severson JJ  et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res 3:620-30 (2015). Human . Read more (PubMed: 25701326) »
  • Jiang J  et al. Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer. PLoS One 8:e81799 (2013). IHC-P ; Human . Read more (PubMed: 24339967) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neurons)
Specification Neurons
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Gokhan Yilmaz

Verified customer

投稿 Sep 21 2016

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Please let me know if you have any success by changing the blocking buffer. It is actually not that uncommon to load such amounts of whole cell lysates, however if that is not feasible for your experiments I am...

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Thank you for contacting Abcam regarding ab69630.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to impro...

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Thank you for your reply.


We did not test this antibody with a standard protein.This antibody can be used for ELISA due to validation by the following:


1) Peptide-Based ELISA: Coat immunogen peptide in 96-well plate. Det...

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Thank you once again for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As requested, I have obtained a copy of the testing protocol. Please bear in mind that this...

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Thank you for contacting Abcam regarding ab69630.


Regarding our testing conditions for ELISA, this was tested for immunogen-coated ELISA using a conjugated secondary antibody. It has not been tested in sandwich ELISA.


Th...

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Thank you for your enquiry. I have contacted the originator of the product, but unfortunately, there is only limited amount of the peptide and is not available for purchase. I am sorry I can't be of more assistance in this occasion but if there...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"