製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Galectin 8
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Human, Cynomolgus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human galectin 8.

    (Peptide available as ab42878.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Galectin 8 Tagged Recombinant Protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 70 kDa corresponding to the molecular weight of the Galectin 8 tagged recombinant protein (predicted molecular weight of Galectin 8: 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • Anti-Galectin 8 antibody (ab42879) at 1 µg/ml + Galectin 8 Recombinant Protein at 0.1 µg

      Secondary
      Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 35 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    参考文献

    ab42879 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab42879.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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