製品の概要

  • 製品名Anti-Galectin 7 antibody
    Galectin 7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Galectin 7
  • アプリケーション適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Lectin, Galactoside-binding 7 encoded within exon 4 (LocusLink ID 3963).

  • ポジティブ・コントロール
    • Modified RIPA extract from the epidermis of FVB wild-type or galectin 7 transgenic mice.
  • 特記事項


    The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were affinity purified using the peptide immobilized on solid support.
  • 一次抗体 備考The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
IP Use at 10-20 µg/mg of lysate.
ICC/IF 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
  • 組織特異性Mainly expressed in stratified squamous epithelium.
  • 配列類似性Contains 1 galectin domain.
  • 細胞内局在Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gal-7 antibody
    • GAL7 antibody
    • Galectin 7B antibody
    • Galectin-7 antibody
    • HKL-14 antibody
    • Human keratinocyte lectin 14 antibody
    • Keratinocyte lectin 14 antibody
    • Lectin galactoside binding soluble 7 antibody
    • Lectin, galactoside binding, soluble, 7B antibody
    • LEG7_HUMAN antibody
    • LGALS7 antibody
    • LGALS7A antibody
    • LGALS7B antibody
    • P53 induced protein 1 antibody
    • p53-induced gene 1 protein antibody
    • Pi7 antibody
    • PIG1 antibody
    • TP53I1 antibody
    see all

Anti-Galectin 7 antibody 画像



  • Predicted band size : 16 kDa


    ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10 µg) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20 µg) from the epidermis of FVB wild-type mice. Antibody: ab10482 used at 0.005 (A) or 0.0025 (B) µg/ml for WB and as indicated for IP. Detection: Chemiluminescence with 1 second exposure time.
  • ab10482 staining Galectin7 in Human cervical tumor and immortalized keratinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS and blocked with 1% BSA for 1 hourat 25°C. Samples were incubated with primary antibody (1/1000 in 1% BSA/PBS) for 24 hours at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
    SiHa cells (negative control - very low Gal-7 mRNA)
    Immortilized keratinocytes (positive control - high Gal-7 mRNA)
  • IHC image of ab10482 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10482, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Galectin 7 antibody (ab10482) 使用論文

This product has been referenced in:
  • Treutlein G  et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . Read more (PubMed: 23236492) »
  • Honoré B  et al. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci 6:18 (2008). WB . Read more (PubMed: 18544163) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Keratinocytes)
Specification Keratinocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton-x-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Keratinocytes)
Loading amount 20 µg
Specification Keratinocytes
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (schwannoma cell lines, LAN1, U87)
Loading amount 75 µg
Specification schwannoma cell lines, LAN1, U87
Gel Running Conditions Reduced Denaturing (12.5-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cervical tumor and immortalized keratinocytes)
Specification cervical tumor and immortalized keratinocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 / PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (keratinocytes and cervical tumors)
Loading amount 80 µg
Specification keratinocytes and cervical tumors
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Bladimiro Rincon Orozco

Verified customer

投稿 Jun 30 2010

Thank you for your enquiry regarding ab10482 and for providing some further information. I am very sorry to hear that you are having some problem with this antibody. I understand from your e-mail that you used lymfoblast cell lysate. Can you please ...

Read More

Thank you for your enquiry and your patience. Here we are forwarding the protocol for Preparation of Epidermal Lysates: Transgenic and nontransgenic mice were killed by cervical dislocation. The dorsal skins were treated with a depilatory agent...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"