Anti-Galectin 7 抗体 (ab10482)
Key features and details
- Rabbit polyclonal to Galectin 7
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Galectin 7 antibody
Galectin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Galectin 7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Galectin 7 aa 100 to the C-terminus.
Database link: 3963 -
特記事項
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
一次抗体 備考
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10482の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).
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ICC/IF | (2) |
Use at an assay dependent concentration.
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IP |
Use at 10-20 µg/mg of lysate.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/10000 - 1/40000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at 10-20 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. -
組織特異性
Mainly expressed in stratified squamous epithelium. -
配列類似性
Contains 1 galectin domain. -
細胞内局在
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 3963 Human
- Entrez Gene: 653499 Human
- Entrez Gene: 16858 Mouse
- Omim: 600615 Human
- SwissProt: P47929 Human
- SwissProt: O54974 Mouse
- Unigene: 558355 Human
- Unigene: 707031 Human
see all -
別名
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
see all
画像
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ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10
ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10 µg) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20 µg) from the epidermis of FVB wild-type mice. Antibody: ab10482 used at 0.005 (A) or 0.0025 (B) µg/ml for WB and as indicated forµ g) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20µ g) from the epidermis of FVB wild-type mice. Antibody: ab10482 used at 0.005 (A) or 0.0025 (B)µ g/ml for WB and as indicated for IP. Detection: Chemiluminescence with 1 second exposure time.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab10482 は 9 報の論文で使用されています。
- Umayahara T et al. Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis. Clin Exp Allergy N/A:N/A (2020). PubMed: 32474952
- Trebo A et al. High Galectin-7 and Low Galectin-8 Expression and the Combination of both are Negative Prognosticators for Breast Cancer Patients. Cancers (Basel) 12:N/A (2020). PubMed: 32290551
- Lin CY et al. Autophagy Receptor Tollip Facilitates Bacterial Autophagy by Recruiting Galectin-7 in Response to Group A Streptococcus Infection. Front Cell Infect Microbiol 10:583137 (2020). PubMed: 33425778
- Lionnard L et al. TRIM17 and TRIM28 antagonistically regulate the ubiquitination and anti-apoptotic activity of BCL2A1. Cell Death Differ N/A:N/A (2018). PubMed: 30042493
- Advedissian T et al. E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface. Sci Rep 7:17086 (2017). PubMed: 29213102
- Monari E et al. Analysis of protein expression in periodontal pocket tissue: a preliminary study. Proteome Sci 13:33 (2015). WB . PubMed: 26719749
- Gendronneau G et al. Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair. PLoS One 10:e0119031 (2015). PubMed: 25741714
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492
- Honoré B et al. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Proteome Sci 6:18 (2008). WB . PubMed: 18544163