The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105 cells. ab95679 can be used at 0.25 µg/per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Contains 1 galectin domain.
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.