製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Galectin 3
  • 特異性
    Galectin 3 antibody detects endogenous levels of total Galectin 3 protein.
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Galectin 3 (internal sequence). The immunogen is in the range of aa 141-190.
    Database link: P17931

  • ポジティブ・コントロール
    • human lung carcinoma tissue; Hela cell extract

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 21652631
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 組織特異性
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 配列類似性
    Contains 1 galectin domain.
  • 細胞内局在
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Anti-Galectin 3 antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).
  • All lanes : Anti-Galectin 3 antibody (ab53082) at 1/500 dilution

    Lane 1 : Hela cell extract with no blocking peptide
    Lane 2 : Hela cell extract with blocking peptide (ab53082 immunogen)


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunocytochemical analysis of mouse macrophage cells, labeling Galectin 3 with ab53082. Cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked in 1% BSA for 30 minutes at 37°C. Incubation with ab53082 (diluted 1/500 in PBS) was for 1 hour at 37°C. The secondary antibody used was ab150073 diluted 1/500.

    See Abreview

Anti-Galectin 3 antibody (ab53082) 使用論文

This product has been referenced in:
  • ElMokh O  et al. Combined MEK and Pi3'-kinase inhibition reveals synergy in targeting thyroid cancer in vitro and in vivo. Oncotarget 8:24604-24620 (2017). IHC-P ; Mouse . Read more (PubMed: 28445948) »
  • Volarevic V  et al. Gal-3 regulates the capacity of dendritic cells to promote NKT-cell-induced liver injury. Eur J Immunol 45:531-43 (2015). Read more (PubMed: 25359399) »

See all 9 Publications for this product

Product Wall

Abreviews
Application
Blocking
Sample
Human Cell (colorectal carcinoma)
Specification
colorectal carcinoma
Username

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Verified customer

投稿 Oct 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Mouse Cell (Macrophage)
Specification
Macrophage
Permeabilization
Yes - Tx100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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