ab38328 gave a positive signal in the following Human Lysates: Heart, Skeletal Muscle, Kidney (Data not shown), Thymus (Data not shown), HeLa, A431, HL60 (Data not shown), Ramos (Data not shown) and THP1 (Data not shown).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Contains 1 galectin domain.
Secreted > extracellular space > extracellular matrix.
All lanes : Anti-Galectin 1 antibody (ab38328) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa Observed band size: 14 kDa Additional bands at: 34 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Stegemann C et al. Proteomic identification of matrix metalloproteinase substrates in the human vasculature. Circ Cardiovasc Genet6:106-17 (2013).
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Woolley JF & Al-Rubeai M The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture. Biotechnol Bioeng104:590-600 (2009).
Read more (PubMed: 19544303) »