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Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
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Galactose (C6H12O6 MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of a-D-galactose and ß-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.
|Galactose Assay Buffer||WM,||1 x 25ml|
|Galactose Enzyme Mix (Lyophilized)||Green||1 vial|
|Galactose Probe (DMSO solution)||Red||1 x 200µl|
|Galactose Standard (100 nmol/ul)||Yellow||1 x 100µl|
|HRP (Lyophilized)||Purple||1 vial|
Our Abpromise guarantee covers the use of ab83382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.
Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.
Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.
Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.
ab83382 has not yet been referenced specifically in any publications.