製品の概要

  • 製品名Galactose Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    < 10 nmol/well
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Galactose (C6H12O6 MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of a-D-galactose and ß-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 関連性Galactose (C6H12O6 FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes.

アプリケーション

Our Abpromise guarantee covers the use of ab83382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Galactose Assay Kit 画像

  • Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.

    Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.

  • Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.

  •  Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.

  • Example of Galactose standard curve obtained with ab83382

プロトコール

Galactose Assay Kit (ab83382) 使用論文

ab83382 has not yet been referenced specifically in any publications.

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I can confirm that this kit (ab83382: Galactose Assay Kit) will measure only galactose. The detection range which your customer has is not associated with this kit. All we mention on the product datasheet is that this ki...

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Thank you for your enquiry.
The ab83382 assay is for galactose. There is not going to be any measurement of enzyme activity in this kit. This kit will measure the total amount of free galactose in your samples.
If you need any further assista...

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Thank you for your patience with this request.

The datasheet team added this standard as ab140312. It should be available on the catalogue in a few days.

I hope this information helps. Please contact us with any other questions.

Thank you for contacting us.


With one Galactose Assay Kit (ab83382) you are provided with enough reagents for a total of 100 tests. As discussed over the phone this does include all the control assays that need to be performed (so takin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"