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Abcam's Galactose and Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (OD570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.
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Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
|Galactose Assay Buffer||WM||1 x 25ml|
|Galactose Enzyme Mix (Lyophilized)||Green||1 vial|
|Galactose Probe (DMSO solution)||Red||1 x 200µl|
|Galactose Standard (100nmol/ul)||Yellow||1 x 100µl|
|HRP (Lyophilized)||Purple||1 vial|
|Lactase (Lyophilized)||Blue||1 vial|
Our Abpromise guarantee covers the use of ab83378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab83378 has not yet been referenced specifically in any publications.