Anti-GAL4 抗体 (ab229347)
Key features and details
- Rabbit polyclonal to GAL4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GAL4 antibody
GAL4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GAL4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human GAL4 (internal sequence). The exact sequence is proprietary.
Database link: P56470 -
ポジティブ・コントロール
- WB: Rat and mouse colon extracts; HCT 116 whole cell extract; GAL4 transfected HEK-293T whole cell lysate. IHC-P: Human gastric cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab229347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
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WB |
1/500 - 1/10000. Predicted molecular weight: 36 kDa.
|
特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 36 kDa. |
ターゲット情報
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機能
Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. - Information by UniProt
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参照データベース
- Entrez Gene: 3960 Human
- Entrez Gene: 16855 Mouse
- Entrez Gene: 25474 Rat
- Omim: 602518 Human
- SwissProt: P56470 Human
- SwissProt: Q8K419 Mouse
- SwissProt: P38552 Rat
- Unigene: 5302 Human
see all -
別名
- Antigen NY CO 27 antibody
- Antigen NY-CO-27 antibody
- Antigen NYCO27 antibody
see all
画像
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Paraffin-embedded human gastric cancer tissue stained for GAL4 using ab229347 at 1/500 dilution in immunohistochemical analysis.
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Anti-GAL4 antibody (ab229347) at 1/5000 dilution + Rat colon extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Anti-GAL4 antibody (ab229347) at 1/500 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Anti-GAL4 antibody (ab229347) at 1/1000 dilution + Mouse colon extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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All lanes : Anti-GAL4 antibody (ab229347) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : GAL4-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab229347 は 1 報の論文で使用されています。
- Ding Y et al. LGALS4 as a Prognostic Factor in Urothelial Carcinoma of Bladder Affects Cell Functions. Technol Cancer Res Treat 18:1533033819876601 (2019). PubMed: 31558111