Anti-GAK protein antibody
Rabbit polyclonal to GAK protein
Synthetic peptide corresponding to Human GAK aa 15-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q6P490
Mouse heart tissue lysate. Human hepatocarcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 62 kDa.
1/50 - 1/100.
GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.
(Information taken form the supplier datasheet for ab86397)
Western blot - GAK antibody - N-terminal (ab86397)
Anti-GAK protein antibody (ab86397) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg
Predicted band size: 62 kDa Observed band size: 62 kDa Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAK antibody - N-terminal (ab86397)
ab86397 at a 1/1000 dilution staining GAK in hepatocarcinoma tissue. DAB staining.
has not yet been referenced specifically in any publications.
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