製品の概要

  • 製品名
    Anti-GAK protein antibody
  • 製品の詳細
    Rabbit polyclonal to GAK protein
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human GAK aa 15-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6P490

  • ポジティブ・コントロール
    • Mouse heart tissue lysate. Human hepatocarcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 62 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 関連性
    GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain. (Information taken form the supplier datasheet for ab86397)
  • 参照データベース

画像

  • Anti-GAK protein antibody (ab86397) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ab86397 at a 1/1000 dilution staining GAK in hepatocarcinoma tissue. DAB staining.

参考文献

ab86397 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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