製品の概要

  • 製品名Anti-GAD67 antibody [K-87]
    GAD67 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [K-87] to GAD67
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    RFRRTETDFSNLFARDLLPA

    , corresponding to amino acids 87-106 of Human GAD67

  • ポジティブ・コントロール
    • In Western Blot, this antibody gave a positive signal in Mouse and Rat Brain Tissue Lysates. GAD67 has been thought to be primarily located in the nerve cell body, but using this new K-87 mAb, GAD67 can now also be detected in dendrites and axons.
  • 特記事項


    ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).
  • ポリ/モノモノクローナル
  • クローン名K-87
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab26116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells.

(methanol fixed cells)
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/1000 - 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998).
WB 1/1000 - 1/20000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-Fr 1/2000.

ターゲット情報

  • 機能Catalyzes the production of GABA.
  • 組織特異性Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 関連疾患Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 配列類似性Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody [K-87] 画像

  • Anti-GAD67 antibody [K-87] (ab26116) at 1/2000 dilution + mouse cerebellum at 0.75 µg

    Secondary
    HRP horse anti-mouse IgG at 1/5000 dilution

    Predicted band size : 67 kDa
  • ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.
  • Immunohistochemical staining of mouse pancreatic islet beta cells after antigen retrieval with ab26116 at a dilution of 1/1000.

  • ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-GAD67 antibody [K-87] (ab26116) 使用論文

This product has been referenced in:
  • Umorin M  et al. Genes in the GABA Pathway Increase in the Lateral Thalamus of Sprague-Dawley Rats During the Proestrus/Estrus Phase. J Cell Physiol 231:1057-64 (2016). Read more (PubMed: 26388520) »
  • Cheng YC  et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). ICC/IF ; Human . Read more (PubMed: 27444754) »

See all 24 Publications for this product

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The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Astatotilapia burtoni Tissue sections (Brain)
Permeabilization Yes - 0.1% Triton
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 50mM citrate buffer
Permeabilization No
Specification mouse brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 06 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample Human Tissue sections (brain)
Specification brain
Permeabilization Yes - Tween
Fixative Formaldehyde
Username

Miss. Regina Vontell

Verified customer

投稿 Mar 09 2015

The isotype control, ab18437 is an excellent choice and will work for your FACs needs. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step 10% Normal Goat Serum + 1% BSA + 0.3% H202 in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Tissue sections (Adult PFC Brain Sections)
Specification Adult PFC Brain Sections
Permeabilization Yes - 0.1% Triton X in TBS for 30 minutes
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 15 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Monkey Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 95 degrees/10 min. BioGenex HK057-5KE buffer, 10X conc.
Permeabilization Yes - 0.3% Triton
Username

Dwight German

Verified customer

投稿 Dec 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain sections)
Specification mouse brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Triton during block and staining
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount 10 µg
Specification mouse brain - post nuclear supernatant
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2012

Thank you for submitting the Abreview.

To claim your discount of $337.00 of your next order, please place the order for the antibody in question via your normal purchasing procedure and include the AbTrial discount code in the order. Our cust...

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