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Synthetic peptide corresponding to a region of GAD67 (EC 220.127.116.11) shared between the mouse (P48318), rat (NP_058703) and human (Q99259) gene products conjugated to KLH.
It is important to note that, in our hands, we saw only a subpopulation of GABAergic neurons in various mouse brain structures, including cerebral cortex and cerebellar cortex, labeled with our anti-GAD-67 antibody. This was seen multiple times, and following steps to increase antibody penetration into the tissue. Although the basis of this mysterious subpopulation is unclear, we suspect that the epitope specifically recognized by this antibody on GAD-67 may be cryptic or absent in some inhibitory neurons.
Our Abpromise guarantee covers the use of ab75712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/2000.|
|IHC-P||1/1000 - 1/2000.|
Immunocytochemistry/Immunofluorescence analysis of e18 mouse neurosphere cells in culture labelling GAD67 (green) with ab75712. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue: DAPI nuclear stain.
ab75712 has not yet been referenced specifically in any publications.