製品の概要

  • 製品名Anti-GAD67 antibody
    GAD67 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to GAD67
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a region of GAD67 (EC 4.1.1.15) shared between the mouse (P48318), rat (NP_058703) and human (Q99259) gene products conjugated to KLH.

  • 特記事項

    It is important to note that, in our hands, we saw only a subpopulation of GABAergic neurons in various mouse brain structures, including cerebral cortex and cerebellar cortex, labeled with our anti-GAD-67 antibody. This was seen multiple times, and following steps to increase antibody penetration into the tissue. Although the basis of this mysterious subpopulation is unclear, we suspect that the epitope specifically recognized by this antibody on GAD-67 may be cryptic or absent in some inhibitory neurons.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/2000.
IHC-P 1/1000 - 1/2000.

ターゲット情報

  • 機能Catalyzes the production of GABA.
  • 組織特異性Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 関連疾患Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 配列類似性Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of e18 mouse neurosphere cells in culture labelling GAD67 (green) with ab75712. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue: DAPI nuclear stain.

Anti-GAD67 antibody (ab75712) 使用論文

ab75712 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry free floating
Sample Zebra finch Tissue (Finch tissue sections (brain))
Specification Finch tissue sections (brain)
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投稿 Sep 13 2016

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Mar 25 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Rat Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.3% trition
Fixative Paraformaldehyde
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投稿 Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Chicken Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.1% titon
Fixative Paraformaldehyde
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投稿 Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Guinea pig Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.2% triton
Fixative Paraformaldehyde
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投稿 Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Guinea pig Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step None
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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投稿 Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Oct 15 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"