製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GAD67
  • 特異性
    ab52249 detects endogenous levels of total GAD67 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human GAD67.

  • ポジティブ・コントロール
    • LOVO cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能
    Catalyzes the production of GABA.
  • 組織特異性
    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 関連疾患
    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 配列類似性
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

画像

  • All lanes : Anti-GAD67 antibody (ab52249) at 1/300 dilution

    Lane 1 : LOVO cell extract; without peptide
    Lane 2 : LOVO cell extract; with peptide


    Predicted band size : 67 kDa
    Observed band size : 67 kDa

プロトコール

参考文献

This product has been referenced in:
  • Nisar R  et al. Diquat causes caspase-independent cell death in SH-SY5Y cells by production of ROS independently of mitochondria. Arch Toxicol 89:1811-25 (2015). WB . Read more (PubMed: 25693864) »
  • Zhang X  et al. The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice. PLoS One 10:e0145638 (2015). WB . Read more (PubMed: 26716999) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (dorsl raphe coronal brain slices)
Specification
dorsl raphe coronal brain slices
Permeabilization
Yes - Triton-X 100
Fixative
Paraformaldehyde
Username

Jason Teem

Verified customer

投稿 Nov 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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