製品の概要

  • 製品名Anti-GAD67 antibody
    GAD67 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GAD67
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-PDSPQRREKLHK

    , corresponding to internal sequence amino acids 526-537 of Human GAD1 (NP_000808.2).

  • ポジティブ・コントロール
    • Human Brain (Cerebellum); Mouse and Rat Brain lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Catalyzes the production of GABA.
  • 組織特異性Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 関連疾患Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • 配列類似性Belongs to the group II decarboxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody 画像

  • Anti-GAD67 antibody (ab104561) at 1 µg/ml + Mouse Brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 67 kDa
  • ab104561 at 4 µg/ml, staining GAD1 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum by Immunohistochemistry using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GAD67 antibody (ab104561) 使用論文

ab104561 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 10% donkey serum and 1% BSA in PBS with 0.1% TritonX -100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Sodium citrate buffer pH 6.0
Sample Mouse Tissue sections (Embryonic mouse brain, 5 um thick sections)
Specification Embryonic mouse brain, 5 um thick sections
Permeabilization Yes - 0.3% TritonX-100 in PBS
Fixative Paraformaldehyde
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投稿 May 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"