製品の概要

  • 製品名Anti-GAD65 antibody [GAD-6]
    GAD65 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GAD-6] to GAD65
  • 特異性The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).
  • アプリケーション適用あり: IHC-P, WB, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Full length protein (Rat)

  • エピトープab26113 recognizes an epitope near the C-terminus of GAD65
  • ポジティブ・コントロール
    • mouse brain

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 18467596
WB 1/1000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/100. PubMed: 24246263
ICC/IF Use at an assay dependent concentration. PubMed: 22745816

ターゲット情報

  • 機能Catalyzes the production of GABA.
  • 配列類似性Belongs to the group II decarboxylase family.
  • 翻訳後修飾Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.
  • 細胞内局在Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 65 kDa glutamic acid decarboxylase antibody
    • DCE 2 antibody
    • DCE2 antibody
    • DCE2_HUMAN antibody
    • GAD 2 antibody
    • GAD 65 antibody
    • GAD-2 antibody
    • GAD-65 antibody
    • Gad2 antibody
    • Glutamate decarboxylase 2 (pancreas) antibody
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate Decarboxylase 65 antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamic Acid Decarboxylase 2 antibody
    • Glutamic Acid Decarboxylase 65 antibody
    • MGC161605 antibody
    • MGC161607 antibody
    see all

Anti-GAD65 antibody [GAD-6] 画像

  • Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution + mouse cerebellum at 0.75 µg

    Secondary
    HRP horse anti-mouse IgG at 1/5000 dilution

    Predicted band size : 65 kDa
  • Mouse monoclonal [GAD-6] to GAD65 staining GAD65-containing nerve terminals around cell bodies of Purkinje cells in the rat cerebellar cortex.

Anti-GAD65 antibody [GAD-6] (ab26113) 使用論文

This product has been referenced in:
  • Farooqi MS  et al. Therapeutic plasma exchange and immunosuppressive therapy in a patient with anti-GAD antibody-related epilepsy: quantification of the antibody response. J Clin Apher 30:8-14 (2015). Read more (PubMed: 24961613) »
  • Zhu B  et al. Resveratrol Reduces Myometrial Infiltration, Uterine Hyperactivity, and Stress Levels and Alleviates Generalized Hyperalgesia in Mice With Induced Adenomyosis. Reprod Sci N/A:N/A (2015). Read more (PubMed: 25701840) »

See all 23 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (human hippocampus)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 10 µg
Treatment used protease inhibitors
Specification human hippocampus
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2015

Thank you for the clarification! Unfortunately the blocking peptide is not available for this particular antibody. Please let me know if I can be of further assistance.

Thank you very much for your reply.

I am sorry to hear that the replacement ab112497 did not work in Western blot. Just to confirm, there were multiple bands in your neuron lysate, and no bands with the rab brain lysate? Were you using the sa...

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Thank you very much for your reply.

I'm sending a free of charge vial of ab112497 on the order **** so that you can compare the results to those with ab26113. This antibody is out of stock but we expect it to arrive around September 27 (next ...

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Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

I actually could not find a reference showing analysis of endogneous GAD65 in culture neurons by Western blot. So, I'm still not quite sure...

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Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. W...

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Thank you for your enquiry regarding ab26113.

It has been reported that GAD65 is transported to nerve terminals and this antibody (ab26113) should stain them as “puncta” around the cell body of their target neurons.

On t...

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Thank you for contacting Abcam.

Based on the protocol the customer sent, there are a few suggestions that may help resolve the issue:

1 - Increase the concentration of primary antibody used to 1/200.

2 - Increase the incub...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"