製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GABRR2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 144-193 (SNKSMTFDGR LVKKIWVPDV FFVHSKRSFT HDTTTDNIML RVFPDGHVLY) of human GABRR2 (NP_002034).

  • ポジティブ・コントロール
    • Hela cell lysate IF/ICC: SKNSH cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性
    GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the gaba/benzodiazepine receptors and opening an integral chloride channel. GABRR2 is a member of the rho subunit family of GABA receptors and could play a role in retinal neurotransmission.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • GABA receptor Rho 2 subunit antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit rho-2 antibody
    • GABA(C) receptor antibody
    • GABRR 2 antibody
    • Gamma aminobutyric acid receptor rho 2 subunit [Precursor] antibody
    • Gamma-aminobutyric acid (GABA) A receptor, rho 2 antibody
    • Gamma-aminobutyric acid receptor subunit rho-2 antibody
    • Rho 2 GABA receptor antibody
    see all

画像

  • ICC/IF image of ab83223 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83223, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-GABRR2 antibody (ab83223) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    Gel Concentration: 12%

参考文献

This product has been referenced in:
  • Rosas-Arellano A  et al. A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays. Histochem Cell Biol 146:421-30 (2016). Read more (PubMed: 27188756) »
  • Fatemi SH  et al. mRNA and protein expression for novel GABAA receptors ? and ?2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway. Transl Psychiatry 3:e271 (2013). WB ; Human . Read more (PubMed: 23778581) »

See all 2 Publications for this product

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