Anti-GABPB2/Nuclear respiratory factor 2 抗体 (ab88746)
Key features and details
- Mouse polyclonal to Nuclear respiratory factor 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GABPB2/Nuclear respiratory factor 2 antibody -
製品の詳細
Mouse polyclonal to Nuclear respiratory factor 2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein within Human GABPB2/Nuclear respiratory factor 2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. -
配列類似性
Contains 5 ANK repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2553 Human
- Entrez Gene: 499883 Rat
- Omim: 600610 Human
- SwissProt: Q06547 Human
- Unigene: 654350 Human
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別名
- BABPB2 antibody
- E4TF1 antibody
- E4TF1-47 antibody
see all
画像
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Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg
Predicted band size: 42 kDa -
Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg
Predicted band size: 42 kDa -
Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 42 kDa -
All lanes : Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml
Lane 1 : lysate from transfected 293T cells
Lane 2 : lysate from non-transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 42 kDa -
ab88746 at 3 µg/ml staining Nuclear respiratory factor 2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
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ab88746 at 10 µg/ml staining Nuclear respiratory factor 2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab88746 は 2 報の論文で使用されています。
- Hwang JH et al. TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A. Nat Commun 13:653 (2022). PubMed: 35115527
- Gallart-Palau X et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep 5:14664 (2015). PubMed: 26419333